IN VITRO CLONAL PROPAGATION OF ONION (ALLIUM CEPA L.) THROUGH SHOOT TIP CULTURE

The aim of the present investigation was to standardize in vitro shoot tip culture technique considering various aspects for direct plant formation two onion (Allium cepa L.) varieties ‘Indian’ and ‘Taherpuri’. About 1.5-2.2 mm tips were collected from bulbs sterilized with 0.1% HgCl2 duration. Then they cultured on MS medium supplemented hormonal concentrations different media composition primary establishment. BAP 1.5 mg l-1 + NAA1.0 found be best formulation establishment tips. Primarily established further multiplication, 2ip 2.0 NAA 0.5 most effective multiplication. highest percentage shootlet produced root MS+2.0 IBA both cultivars (Taherpuri 90% Indian 80%). plantlets transferred small pots containing soil : sand 1:1 kept under shade covered perforated polythene sheet. After proper hardening field.